Mean of three absorbance values of the standard mixture and the formulation sample at 4�C20 ��g mL-1 for DRT and 4.5�C22.5 ��g Tubacin microtubule mL-1 for ETR were compared by the t-test. Calculated t values (tcal = 1.563) where values were less than the tabulated t (ttab, 2.785) values for both the analytes and this proves that there is no significant difference between the standard mixture of drugs and the formulation sample and thus the specificity of method. Overlay spectra of the standard mixture and formulation solution is similar as shown in Figure 1, which further proves the specificity of the method [Table 4]. Table 4 Specificity study STATISTICAL COMPARISON OF THE RESULTS The results of the baseline manipulation method were compared with the laboratory developed first order-derivative method (FD), ratio derivative (RD), and absorption corrected (AC) methods (unpublished data).

The results of ANOVA for DRT are shown in Table 5. Calculated F values (Fcal) were determined by MIP Pharmasoft 1.0, and these values for both the analytes were less than tabulated F (Ftab) values. As the Fcal values are less than the Ftab values for both drugs it can be concluded that there is no significance difference among these methods and hence the baseline manipulation method is equivalent to these three methods [Table 6]. Table 5 Comparison of results by one way ANOVA Table 6 ANOVA table for drotaverine CONCLUSIONS The newly developed UV spectrophotometric baseline manipulation method was found to be simple, sensitive, accurate, precise, and specific and can be used for the routine quality control analysis of ETR and DRT in combination.

The same concept can be extended for quantitative analysis of other binary and ternary combinations of the analytes in pharmaceuticals. As the method could effectively separate the drugs from each other in a single spectrometric scan, it reduces human efforts and errors as well. ACKNOWLEDGMENTS The authors would like to thank Alkem Laboratories (Mumbai, India), Mapro Pharmaceuticals Ltd., Vapi, JPLC Pharma Ltd. Jalgaon, for providing gift samples of drugs. The authors are also thankful to the Management of MAEER’s Maharashtra Institute of Pharmacy, Pune, for providing necessary Dacomitinib facilities. Footnotes Source of Support: Nil. Conflict of Interest: None declared.
Chemically cefpodoxime proxetil (CEFPO) [Figure 1] is (6R,7R)-7-[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetamido]-3-(methoxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid. It is an oral third generation cephalosporin antibiotic. It is active against most gram positive and gram negative bacteria.