VpRFP1 was initially identified as an expressed sequence tag (EST) from

a cDNA library constructed from leaves of V. pseudoreticulata inoculated with the grapevine powdery mildew Uncinula necator. Sequence analysis of the deduced VpRFP1 protein STI571 cell line based on the full-length cDNA revealed an N-terminal nuclear localization signal (NLS) and a C-terminal C4C4-type RING finger motif with the consensus sequence Cys-X(2)-Cys-X(13)-Cys-X(1)-Cys-X(4)-Cys-X(2)-Cys-X(10)-Cys-X(2)-Cys. Upon inoculation with U. necator, expression of VpRFP1 was rapidly induced to higher levels in mildew-resistant V. pseudoreticulata plants. In contrast, expression of VpRFP1 was downregulated in mildew-susceptible MEK162 V. vinifera plants. Western blotting using an antibody raised against

VpRFP1 showed that VpRFP1 was also induced to higher levels in V. pseudoreticulata plants at 12-48 hours post-inoculation (hpi). However, there was only slight increase in VpRFP in V. vinifera plants in the same time frame, even though a more significant increase was observed at 96-144 hpi in these plants. Results from transactivation assays in yeast showed that the RING finger motif of VpRFP1 exhibited some activity of transcriptional activation; however, no activity was seen with the full-length VpRFP1. Overexpression of VpRFP1 in Arabidopsis plants was found to enhance resistance to Arabidopsis powdery mildew Golovinomyces cichoracearum, which seemed to be correlated with increased transcript levels of AtPR1 and AtPR2 in the pathogen-infected tissues. In addition, the Arabidopsis transgenic lines showed enhanced resistance to a virulent bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Taken together, the results suggested that VpRFP1 may be a transcriptional activator of defence-related genes in grapevines.”
“BioAggregate

is a new product that was formulated for root-end filling, perforation repair, and pulp capping. This study examined the chemical differences between white mineral trioxide aggregate (MTA) and BioAggregate in both powder and set forms using X-ray diffraction. The results showed that white MTA and BioAggregate have a similar chemical composition Crenolanib clinical trial with some differences: BioAggregate contains a significant amount of tantalum oxide instead of bismuth oxide. In both groups, similar peaks were observed in the set and powder form, but sharper and stronger peaks were observed in the powder samples. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 109: 155-158)”
“Effects of alkalization, acetylation, and maleic anhydride grafted polypropylene (MAPP) treatments on the thermal and chemical properties of the wheat straw fiber were investigated using thermogravimetric analysis, infrared spectrophotometer, X-ray diffraction, and scanning electric microscopy techniques.