These studies sug gested that there may be clinical advantage independent of antibiotic susceptibility pattern. We hypothesized that combination therapy with azithromycin and ampicillin against an isolate resistant to both the drugs might have the ability to exert its bactericidal and anti inflammatory activities independent of antibiotic susceptibility pattern. While results from in vitro research are readily available, in vivo stud ies has to be conducted to confirm the effectiveness of these combination therapy strategies against isolates re sistant to generally advised regimens. SP isolates that had been previously examined in human experimental colonization research also colonized in inbred adult mice, thereby demonstrating the relevance of an animal model of pneumococcal pneumonia.
The aim of your present study was to test the in vivo efficacy of ampicillin and azi thromycin alone and in mixture against a multi drug resistant strain of SP in an experimental murine pneumo nia model. Now the question, regardless of whether a mixture of ampicillin and azithromycin inhibitor P22077 would be powerful in treat ment against an isolate of SP non susceptible to penicillin and macrolide requirements additional investigation in vivo. More over, studying the experimental pharmacodynamic out come connected together with the mixture therapy and evaluating the role of the physiological markers of inflam mation like the level of proinflammatory and anti inflammatory cytokine IL 10 inside the serum following mixture antibiotic therapy, estimating the myeloperoxidase enzyme activity in the web site of infection plus the amount of expression of cyclooxygenase 2 in the lung tissue would also help to elucidate the mechanisms re sponsible for susceptibility to and pathophysiology of lung infection and regulation of these markers in inflammation.
Strategies Antimicrobial agents, media and bacterial strains The study drugs which included ampicillin, selelck kinase inhibitor azi thromycin, amoxicillin potassium clavulanate, oxacillin, ceftazidime, cefotaxime, cefuroxime, ceftriaxone, clindamycin, imipenem, meropenem, levofloxacin, ciprofloxacin, rifampicin, vancomycin, trimethoprim sulphamethoxazole, ce fepime and gentamicin, had been utilized for all in vitro testing as per Clinical and Laboratory Standards Institute guidelines as well as the exact same AMP and AZM were utilized for intravenous injection in mice. The clinical isolate of S.
pneumoniae, AMRI SP 1, utilised for the experiment was obtained from the sputum of a patient with lower respiratory tract infection, admitted to Sophisticated Medicare and Analysis Institute hospital in Kolkata, West Bengal, India. A high quality manage strain of SP, ATCC 49619 was obtained as a sort gift from Dr. Indranil Roy, The Calcutta Health-related Research Institute, West Bengal, India. The strains have been stored in skimmed milk tryptone glucose glycerol medium at 80 C and subcultured twice onto Columbia blood agar plates supplemented with 5% sheep blood more than night at 37 C in 10% CO2 air incubator before use in all in vitro and in vivo experiments.