The norms cover a maximum age of 12 years, 11 months; therefore, we used data from a larger control sample to convert raw scores to standard scores (see Barry et al., 2007). Handedness was assessed using a brief demonstration hand preference (based
upon the Edinburgh Handedness Inventory; Oldfield, 1971). Children were asked to demonstrate how they would perform each of 10 actions; write, BIBW2992 order draw, throw, use scissors, brush their teeth, cut with a knife, use a spoon, sweep with a broom (upper hand), take the lid of a box, and deal cards. Left, Right, or either (if child indicated both) hand was recorded in each case. The number of right hand preferences was taken as a measure of hand dominance. MRI data were obtained using a 1.5-T Siemens Sonata scanner with a single-channel head coil. Participants wore noise-attenuating headphones and padding was inserted around the head to restrict movement. They watched a DVD of their choice via a mirror on the head coil during acquisition of the structural data. A T1-weighted image was acquired in each participant Panobinostat clinical trial for structural analysis and image registration (3D
FLASH; TR = 12 ms; TE = 5.6 ms; 1 mm isotropic voxels; matrix = 256 × 256 × 208; elliptical sampling; orientation = coronal). One acquisition of this T1-weighted sequence took five minutes. At the end of these five minutes, the image was inspected for motion artefacts and, if necessary, children were reminded to keep still for a further five minutes. Three artefact-free images were successfully acquired in each participant. The first and third images were registered (rigid-body transformation; 6 degrees of freedom; trilinear interpolation) to the second image to correct for movement Inositol monophosphatase 1 between acquisitions and summed to create a single T1-weighted
image in each participant. Before the functional task, participants were removed from the scanner for a break if necessary. For the functional scan, whole-head T2∗-weighted echo-planar images (TR = 3s; TE = 50 ms; 120 volumes, 6 min), were acquired. Each volume comprised 35 4-mm axial slices (in-plane resolution 3 mm × 3 mm). Stimuli were presented over MRI compatible headphones (MR Confon: http://www.mr-confon.de) at a comfortable listening level (estimated ∼70 dB). Participants were asked to keep their eyes closed. The task used for functional imaging was based on the Auditory Responsive Naming task previously used with PET (Bookheimer et al., 1998). It was chosen because it was thought to be engaging for children, easy enough for them to comply with and known to produce activation in both posterior and anterior language areas (Wernicke’s and Broca’s area, respectively). In the Speech condition, participants heard simple three-word auditory definitions of a high frequency word and were required to silently generate an appropriate word; for example, ‘wear on head’ > silently generate ‘hat’.