The diversity of blaZ gene as measured by the Simpson index of diversity (SID) was higher for the MRSA collection than for MSSA, although PND-1186 clinical trial not statistically significant
due to the partial overlapping of the confidence intervals (SID = 79.18, 95%CI 69.6-88.8 vs SID = 76.09, 95%CI 61.3-90.9, respectively) – see Table 4. Within the length of blaZ region analyzed (492 nucleotides), we Transmembrane Transporters inhibitor detected 43 unique single-nucleotide polymorphisms (SNP) and on average, each blaZ allele has 12.4 SNP comparing to the prototype blaZ sequence of Tn552 (allele 1) – see Tables 3 and 4. Overall, blaZ alleles were more variable in MSSA than in MRSA (14.7 and 11.4 SNP/allele, respectively). As illustrated by the allelic frequency distribution
per MRSA lineage (Figure 1) or the cluster tree of the thirteen blaZ alleles found in our collections (Figure 2), there is no clustering according to genetic lineages, as defined by MLST sequence type and SCCmec type, or MSSA/MRSA phenotype; i.e. In addition, there was also no clear clustering of blaZ allotypes according to geographic origin or isolation date of the MRSA learn more isolates (see Table 1). Frequency SNPc) Amino acid substitutions MRSA a) MSSA b) Silent Conservative Missense Nonsense 1 0.43 0.21 0 0 0 0 0 2 0.02 0 1 0 0 1 1 3 0.07 0.04 9 4 2 2 0 4 0.04 0 9 4 2 3 0 5 0.06 0 7 2 2 3 0 6 0.11 0.46 13 8 2 3 0 blaZ 7 0.02 0 12 6 2 4 0 8 0.10 0.04 11 6 2 3 0 9 0.07 0.08 20 9 2 7 0 10 oxyclozanide 0.04 0.04 19 8 2 7 0 11 0.06 0.04 24 11 3 8 0 12 0 0.04 24 11 2 8 0 13 0 0.04 12 7 2 3 0 1 0.33 0.45 0 0 0 0 0 2 0.15 0.25 6 5 0 1 0 3 0.19 0.15 1 0 0 1 0 4 0.19 0.05
4 3 0 1 0 blaI 5 0.04 0 7 5 0 2 0 6 0.07 0 4 3 0 1 0 7 0.04 0 5 4 0 1 0 8 0 0.05 3 1 1 1 0 9 0 0.05 1 0 0 1 0 1 0.26 0.24 0 0 0 0 0 2 0.07 0 19 9 4 6 0 3 0.10 0 18 7 4 6 0 4 0.07 0.06 35 15 9 10 0 5 0.07 0.18 35 15 7 11 0 6 0.07 0.12 17 6 4 6 0 blaR1 7 0.07 0.06 24 10 7 7 0 8 0.03 0 33 12 6 12 0 9 0.16 0 31 11 6 11 0 10 0.13 0.24 32 12 6 11 0 11 0 0.06 20 9 5 7 0 12 0 0.06 34 16 6 10 0 a) The total number of MRSA strains whose blaZ, blaI and blaR1 genes were analyzed is 54, 27 and 31, respectively.