Project information is stored in the Genomes OnLine Database [14]. The Whole Genome Shotgun (WGS) sequence is deposited in Genbank LY188011 and the Integrated Microbial Genomes database (IMG) [33]. A summary of the project information is shown in Table 2. Table 2 Genome sequencing project information Growth conditions and DNA isolation A culture of DSM 19593T was grown aerobically in DSMZ medium 514 [34] at 28��C. Genomic DNA was isolated using Jetflex Genomic DNA Purification Kit (GENOMED 600100) following the standard protocol provided by the manufacturer but modified by an incubation time of 60 min, incubation on ice over night on a shaker, the use of additional 50 ��l proteinase K, and the addition of 100 ��l protein precipitation buffer. DNA is available from DSMZ through the DNA Bank Network [35].

Genome sequencing and assembly The genome was sequenced using one Illumina PE library (Table 2). Illumina sequencing [36] was performed on a GA IIx platform with 150 cycles. The paired-end library contained 520 bp insert size. To correct sequencing errors and improve quality of the reads, clipping was performed using fastq-mcf [37] and quake [38]. After this step 4,717,610 reads with a median length of 124 bp were assembled using velvet [39]. The resulting draft genome consisted of 71 contigs organized in 45 scaffolds. The initial draft sequences were separated into artificial Sanger reads of 1,000 nt size plus 75 nt overlap. The number of gaps was reduced by manual editing in phred/phrap/consed version 20.0 [40]. The final assembly was composed of 17 contigs organized in 15 scaffolds.

(The version deposited at Genbank contains two scaffolds less, which did not meet the requirements for the minimal contig length.) The additional fragments ‘thalar_Contig12.1′ and ‘thalar_Contig18_1.4′ can be found in the IMG database).The combined sequences provided a 195�� GSK-3 coverage of the genome. Genome annotation Genes were identified using Prodigal [41] as part of the JGI genome annotation pipeline [42]. The predicted CDSs were translated and used to search the National Center for Biotechnology Information (NCBI) nonredundant database, UniProt, TIGR-Fam, Pfam, PRIAM, KEGG, COG, and InterPro databases. Identification of RNA genes were carried out by using HMMER 3.0rc1 [43] (rRNAs) and tRNAscan-SE 1.23 [44] (tRNAs). Other non-coding genes were predicted using INFERNAL 1.0.2 [45]. Additional gene prediction analysis and functional annotation was performed within the Integrated Microbial Genomes – Expert Review (IMG-ER) platform [33]. CRISPR elements were detected using CRT [46] and PILER-CR [47]. Genome properties The genome statistics are provided in Table 3 and Figure 3. The genome consists of a 3.