Mycophenolate mofetil is available as commercial tablets under the brand name Mycofit 250 mg from Intas Pharmaceuticals Ltd., and Mycofit 250 mg was procured from the local pharmacy. Preparation of the standard stock solution Analyte (10, 20, 30, 40, and 50 mg) Tofacitinib JAK3 was accurately weighed and separately dissolved in methanol in 100 mL volumetric flasks to furnish solutions in the concentration range of 100�C500 ng ��L-1. These solutions were used for the working range. Chromatographic conditions Chromatography was performed on 10 �� 10 cm aluminum plates precoated with 250 ��m layers of silica gel 60 F254 (E. Merck, Darmstadt, Germany). Before use, the plates were prewashed with methanol and activated at 110�� for five minutes.

The samples were applied to the plates as bands that were 6 mm wide and 10 mm apart by means of a Camag Linomat V sample applicator (Camag, Muttenz, Switzerland) equipped with a 100 ��l syringe (Hamilton, Bonaduz, Switzerland). Linear ascending development was performed in a 10 �� 10 cm twin trough glass chamber (Camag), with toluene, acetone, and methanol in the ratio 6:2:2 (v/v/v) as the mobile phase and the chamber was presaturated with mobile phase vapor for 10 minutes. The development distance was 8.5 cm with a development time of approximately 60 minutes. After chromatography, the plates were dried in a current of air by using air-blowing drier. Densitometric scanning was performed with a Camag TLC Scanner 3 at 254 nm for all measurements. The scanner was operated by Wincats software version 1.2.3.

The source of radiation was a deuterium lamp emitting a continuous ultraviolet (UV) spectrum between 200 and 400 nm. The slit dimensions were 5 �� 0.45 mm and the scanning speed was 20 mm s-1. After chromatographic development, the bands were scanned in the range of 200�C400 nm (spectrum scan speed: 20 nm s-1) so that the drug could be estimated at 254 nm, which is ascertained by taking the spectrum at different concentrations between 100 and 500 ng with 100 ng increment. Further, it is also observed that the spectra are similar in their behavior. Procedure for the standard The standard stock solution of mycophenolate mofetil was applied on a TLC plate, (1 ��L) by using the Linomat V sample applicator and the 100 ��l syringe. The plate was developed and scanned under the conditions described above.

Each amount was analyzed five times and peak areas were recorded. A calibration plot of peak area against the respective amount was established for mycophenolate mofetil. Procedure for the sample Twenty tablets were weighed accurately and finely powdered. A quantity of powder equivalent to 10 mg mycophenolate mofetil was weighed and transferred to a 100 mL volumetric flask containing approximately Carfilzomib 50 mL methanol. The mixture was ultrasonicated for five minutes; then, the final dilution was made with methanol.