For this reason, a PCR protocol able to detect and quantify DENV in the different kinds of samples employed for DENV study is of great value. In this study, a real-time PCR method suitable not only for clinical objectives but also for laboratory routine is described. Sequences from several strains of DENV comprising the four serotypes were aligned. A fragment located at the 5′UTR region of the virus genome was used to generate the primers

and the probe for real-time PCR. This method was successfully used to identify and quantify distinct dengue virus strains and serotypes in clinical samples, in sera from patients SBC-115076 chemical structure infected with dengue virus, and in the mosquito Aedes aegypti, as well as to study virus replication in different cell lines. (C) 2009 Elsevier B.V. All rights reserved.”
“Tau phosphorylation and hypoxia are both linked to the pathology of Alzheimer’s disease. To find out the possible connection between hypoxia and tau phosphorylation, we performed this study to evaluate the level of phosphorylated tau under hypoxic or normal condition. We found in our study that hypoxia promoted the phosphorylation of tau protein via ERK pathway, which suggest hypoxia might be involved in the process of tau pathology. (C) 2010 Elsevier Ireland Ltd. All rights reserved.”
“To investigate the structure and function of

nonstructural GSK2879552 molecular weight (NS) protein 2B of the dengue serotype 2 virus (DV2) during infection, polyclonal antibodies (Abs) against DV2 NS2B were prepared by immunisation with NS2B protein or by DNA immunisation. The full-length NS2B gene was cloned and inserted into the prokaryotic expression vector pQE31, resulting in a vector, named pQE-NS2B, or into the eukaryotic expression vector pCAGGS-P7, resulting in the vector pCAG-NS2B. The pQE-NS2B vector was transfected into Escherichia coli JM109, and recombinant NS2B protein was obtained Bortezomib by Ni(2+)-NTA agarose affinity chromatography Vero cells transfected with pCAG-NS2B showed that NS2B protein can be expressed in eukaryotic

cells. Finally, mice were immunised with the recombinant NS2B protein or pCAG-NS2B. Anti-NS2B sera from the immunised mice could specifically react with DV2 NS2B proteins, as visualised by fluorescence staining and Western blotting. Immunisation with NS2B protein induced a higher titre of the antibody than that induced by DNA immunisation. These data indicate that our antisera against DV2 NS2B can recognise both the natural and denatured NS2B protein. Based on these results, the polyclonal Abs could be used as a tool for studying the role of NS2B in the pathogenesis of DV2. (C) 2009 Published by Elsevier B.V.”
“Stem cell research offers enormous potential for treating many diseases of the nervous system. At present, therapeutic strategies in stem cell research segregate into two approaches: cell transplantation or endogenous cell stimulation.