For the remainder of the studies, we focused on the effects

For the remainder of the studies, we focused on the effects

of the tannins against HCMV, HCV, DENV-2, MV, and RSV. Free virus particles are inactivated by CHLA and PUG CHLA and PUG were previously observed to inactivate HSV-1 particles and prevent their interaction with the host cell AMN-107 order surface [33]. We examined whether the tannins could also inactivate the different enveloped viruses and prevent subsequent infection. These natural products were pre-incubated with the viruses and then diluted to sub-therapeutic concentrations prior to infecting the respective host cell. Results indicated that both CHLA and PUG were able to interact with HCMV, HCV, DENV-2, Selleck AZD1152-HQPA MV, and RSV virions. Their effects were irreversible and abrogated subsequent infections (Figure 3). A 60 – 80% block against the paramyxoviruses MV and RSV was observed, whereas near 100% inhibition was achieved against HCMV, HCV, and DENV-2. The data suggest

that CHLA and PUG can directly inactivate these free virus particles and neutralize their infectivity. CHLA and PUG inhibit virus entry-related Selleck ICG-001 steps In further characterizing the antiviral mechanism(s) involved, we explored the effect of CHLA and PUG against HCMV, HCV, DENV-2, MV, and RSV attachment to the host cell surface and upon subsequent membrane fusion. The temperature change between 4°C (permitting virus binding but not entry) and 37°C (facilitating virus entry/penetration) allows examination of the drug effect on each specific event [53]. Both tannin compounds effectively prevented attachment of the investigated viruses as shown by readouts of inhibition of infection (method 1; Figure 4) and by ELISA-based binding assays Teicoplanin using virus-specific antibodies

to detect bound virus on the cell monolayer (method 2; Figure 5). The inhibition of virus attachment by CHLA and PUG were similar against HCMV, HCV, DENV-2, and RSV, and ranged from 90 – 100% (Figure 4). Against MV, PUG appeared to be more effective than CHLA, and inhibition of entry varied between 50 – 80%. The compounds’ ability to abolish binding of the above viruses was confirmed by the decrease of virions detected on cell surfaces. This occurred in a dose-dependent manner with increasing concentrations of the tannins (Figure 5). To see whether the CHLA and PUG retained their activity during the virus penetration phase, the test viruses were allowed to bind to the cell surface at 4°C and then allowed to penetrate the target cell membrane by a temperature shift to 37°C in the presence or absence of the tannins. CHLA and PUG were again observed to impair virus entry by these viruses, resulting in 50 – 90% protection of the host cell from infection from the virus being examined (Figure 4).

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