After incubation, the bacterial cells were washed from the surface of the agar and suspended in sterile 0.1 ml phosphate buffer saline, pH 7.4 and diluted to about 2 × 107 colony forming units (CFU)/ml.

The spreading of bacterial suspension (0.1 ml) seeded the surface of MH agar plates. On the agar surface, holes of 8 mm diameter were punched and 25 μl of phenolic extract of different concentrations (80, 160 and 240 μg) was placed in each well. The plates were incubated overnight at 37 °C, and the zone of inhibition was measured. The experiment was carried out in triplicate and the effect of solvent (methanol) on the microbial growth was also analyzed. A HDAC inhibitor variety of phenolic compounds derived from spices possess bioactive properties which constitute the largest proportion of known natural antioxidants.25 There are many methods available to assess the antioxidant activity and each having its own limitations.26 In this study, we have tested the antioxidant activity of C. carvi phenolic extract using different antioxidant assays and the growth inhibition effect of C. carvi on selected bacteria causing food spoilage to assess the antibacterial activity. The polyphenolic compounds

from defatted C. carvi INCB024360 chemical structure powder were extracted successively with water, 50% ethanol, and 1:1 mixture of 70% aqueous methanol and 70% aqueous acetone, to facilitate extraction of variety of polyphenols and the yield of polyphenols was found to be 8.76, 12.63 and 50.20 mg/g of defatted powder, respectively. Thus, with the above solvent systems, we could extract a number of phenolic acids and flavonols from C. carvi. The DPPH radical scavenging activity of C. carvi phenolic extract and the commercial antioxidants BHA and BHT were determined as shown in Fig. 1. The purple color of the DPPH solution fades rapidly when it encounters proton radical scavengers. The extract was tested in the concentration range of 0.1–2 μg/ml and the activity was observed in a dose dependent

manner. At a concentration of 0.1 μg/ml, the scavenging activity was 13.7%, Suplatast tosilate whereas at 2 μg/ml, the scavenging activity was 84.6%. The IC50 value of C. carvi phenolic extract was found to be 2.7 μg/ml. The superoxide anion is a reduced form of molecular oxygen and plays an important role in the formation of other reactive oxygen species such as hydrogen peroxide, hydroxyl radical or singlet oxygen.27 The C. carvi phenolic extract was tested for superoxide anion radical scavenging activity at different concentrations as shown in Fig. 2. The C. carvi phenolic extract was found to be an effective scavenger of superoxide anion radicals in a dose dependent manner with an IC50 value of 35 μg/ml. In the reducing power assay, the presence of reductants (antioxidants) in tested samples would result in reducing Fe3+/Ferricyanide complex to the ferrous form. The reducing power of C. carvi phenolic extract was determined in comparison with BHA and BHT standards ( Fig. 3).