the process uses sutures that loop around and compress the external corneal limbal area to make rat ocular hypertension, the magnitude of which is dependent upon the weights connected to the ends of the suture. In our research, we characterized the relationship between the applied weights and IOP elevation and the consequences of Cathepsin Inhibitor 1 ocular hypertension on the functional and morphological changes within the retina, thereby damaging retinal factors in a far more selective and controllable fashion. We further evaluated the performance of the process in evaluating a potential neuroprotective agent, an inhibitor of c Jun N terminal kinase. Being an associate of the mitogen-activated protein kinase family, JNK is active in the signal transduction of many different cellular pathways, including apoptosis, inflammation, and carcinogenesis. Phosphorylation of JNK and activation of its signaling cascade have been demonstrated throughout RGC apoptosis in experimental open-angle glaucoma. Ergo, the restriction of this pathway by specific inhibitors may prevent or slow the development of RGC reduction in the present PACG attack type. SP600125 is just a specific, Metastatic carcinoma commonly-used JNK inhibitor. It’s been demonstrated to change neuronal cell death in rat hippocampal Cornu Ammonis 1 caused by transient mind ischemia/reperfusion. In RGC apoptosis induced by N Methyl D aspartic acid or N Methyl D aspartate, the appearance of JNK improved and the apoptotic process was reversed by SP600125. In an initial report, we demonstrated the p JNK pathway was activated by applying IOP of 45 mmHg more than 6 h and was blocked by SP600125 in the ganglion cell layer. Thus, in the current study, we examined whether SP600125 would reduce RGC reduction caused by ocular hypertension. Techniques employed in this investigation Lu AA21004 conformed to the Association for Research in Vision and Ophthalmology resolution about the Use of Animals in Ophthalmic and Vision Research and were accepted by the Animal Care and Use Committee at Shandong University School of Medicine in China. Male Wistar rats weighing 200 250 g were obtained from the Pet Center at Shandong University. They were housed in rooms when the humidity, temperature, and lighting were managed and food and water were available ad libitum. Serious unilateral improved IOP was induced by the suture pulley corneal limbal pressure technique described previously. Shortly, mice were anesthetized with chloral hydrate, with additional doses given as-needed. A line of around 70 cm was linked to the loads at both ends. The line was then looped around the circumference of the eyeball about 2 mm behind the limbus. Circumferential retention of the planet symmetric to the optical axis was made by passing both ends of the suture thread via a number of pulleys. The neglected attention served as a get a grip on.