From the DPPH scavenging assay, leaf extracts of L. indica were investigated through the totally free radical scaven ging activity by way of their reaction with all the steady DPPH radicals. The radical scavenging activity values of the extracts are shown in Table 2. Low EC50 value indi cates robust capability from the extract to act as DPPH scaven ger. A substantial EC50 worth indicates lower scavenging exercise of the scavengers as additional amounts of your scavengers have been necessary to achieve 50% scavenging reaction. This means, the scavengers are much less successful in scavenging the DPPH radicals. Between the four extracts, the fractio nated water extract showed the stron gest scavenging exercise in contrast with all the conventional ascorbic acid, followed by ethanol and ethyl acetate extracts.
The hexane ex tract showed the weakest scavenging exercise on DPPH radicals using the EC50 worth of 1285 ug ml. The existing consequence is consistent together with the preceding information reported by Saha et al, which selleck inhibitor showed that methanol extract from the total plant of L. indica also showed solid no cost rad ical scavenging action comparable with quercetin, BHT and Vitamin C. The results in Table 1 and Table 2 display that there’s a good correlation between higher complete phenolic con tent in the extracts and stronger DPPH scavenging activ ity. This conclusion is supported by published reports which indicated that phenolic substances typically effectively correlated with scavenging activity on DPPH radicals. Consequently, the strong DPPH scavenging exercise of fractionated water extract could possibly be attributed by the higher information of phenolic compounds, al although other antioxidants could current during the fractio nated water extract also.
Lowering power of L. indica leaf extracts Former research have pointed out the inhibitor price antioxidant capability is normally connected to the improvement of reduc tones which can terminate the totally free radical chain reac tions. The minimizing power of L. indica extracts are shown in Table 3. From the minimizing power assay, a more powerful absorbance signifies a increased minimizing electrical power. The extracts that showed comparable absorbance study ings with all the positive reference typical are thought of to possess higher cutting down electrical power. The lowering electrical power of the four extracts varied signifi cantly at distinctive concentrations. The lowering electrical power of every one of the extracts enhanced grad ually together with the improve in concentrations in the extracts.
Normally, the fractionated water extract showed a sig nificantly higher decreasing energy in any respect the examined concentrations in contrast on the other extracts. This was followed by ethanol and ethyl acetate extracts, while the hexane extract showed the lowest lowering power between every one of the examined concentrations.At concentration 0. 8 mg ml, the minimizing electrical power of fractio nated water extract was comparable to that of ascorbic acid.